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A peptide library is a collection of a large number of small peptides of specific length and different sequences, which includes the permutation and combination of various (or most) amino acid sequences in short peptides of this length. The peptide library was first proposed by Greyson et al. in 1986. They believed that the binding or recognition between protein molecules is mainly completed by the interaction between several amino acid residues on the partial peptides. Covalent bond: Although some polypeptides have different sequences from the natural epitope of the antigen, the way they bind to antibodies or ligands is the same. Therefore, such polypeptides with key amino acid residues are called mimotopes. The concept of mimotopes played an important role in the development of peptide libraries. Peptide libraries can provide powerful tools for drug design, protein-protein interactions, and other biochemical and drug research and applications.
Phage display peptide library
One-bead One-compound chemical combination peptide library
DNA-encoded chemical combination peptide library
(1) Custom peptide library based on the known structure
Based on the structure of the known compound, a series of polypeptides are constructed and designed including but not limited to the following methods, and screened for the function and indications of the target compound, in order to obtain new molecules or new structures with the target drug effects.
N-terminal, C-terminal, side-chain modification: acylation, esterification, PEGylation, beta-amino acid substitution, D amino acid substitution, main chain aminomethylation, disulfide bond substitution, conformation locking (helix, beta-sheet), Mirror image peptides, cyclic peptides (end-to-end cyclization, side-chain cyclization), special molecular markers, hydroxy acid substitution
(2) Phage display peptide library
Artificially synthesize genes encoding random polypeptide segments, then clone these genes into phage vectors, and display the polypeptides one by one on the surface of the phage through phage epitope display technology to construct a polypeptide library. When screening, based on the specific interaction between a specific receptor target and certain peptides in the peptide library, the peptides that bind to the specific target are screened from the library.
(3) One-bead One-compound chemical combination peptide library
A combinatorial library is a peptide library in which tens of thousands of peptides are synthesized by random synthesis of peptides on the resin. For example, a 5-peptide library would have 5⁵ pentapeptides, and a 9-peptide library would have 9⁹ nonapeptides. Then, during screening, based on the specific interaction between the specific receptor target and certain peptides in the peptide library, the peptides that bind to the specific target are screened out.
(4) DNA-encoded chemical combination peptide library
Polypeptides are randomly synthesized on the carrier resin and also contain a peptide library composed of thousands of polypeptides and DNA synthesized by DNA. Each amino acid will be synthesized corresponding to several nucleotides to encode the information of the polypeptide. When screening, the receptor target needs to be connected to a fluorescent molecule. If a peptide binds to the receptor, the corresponding resin will have fluorescence. Then use these fluorescent resins as templates for PCR amplification, and then sequence to obtain peptide information.
Phage display peptide library:
Artificially synthesize genes encoding random polypeptide segments, then clone these genes into phage vectors, and display the polypeptides one by one on the surface of the phage through phage epitope display technology to construct a polypeptide library. When screening, based on the specific interaction between a specific receptor target and certain peptides in the peptide library, the peptides that bind to the specific target are screened from the library.
One-bead One-compound chemical combination peptide library:
Combinatorial library is a peptide library in which tens of thousands of peptides are synthesized by random synthesis of peptides on the resin. For example, a 5-peptide library would have 55 pentapeptides, and a 9-peptide library would have 99 nonapeptides. Then, during screening, based on the specific interaction between the specific receptor target and certain peptides in the peptide library, the peptides that bind to the specific target are screened out.
DNA-encoded chemical combination peptide library:
Polypeptides are randomly synthesized on the carrier resin and also contain a peptide library composed of thousands of polypeptides and DNA synthesized by DNA. Each amino acid will be synthesized corresponding to several nucleotides to encode the information of the polypeptide. When screening, the receptor target needs to be connected to a fluorescent molecule. If a peptide binds to the receptor, the corresponding resin will have fluorescence. Then use these fluorescent resins as templates for PCR amplification, and then sequence to obtain peptide information.
With an advanced fusion of biotechnology and chemistry, we have successfully established a cyclic peptide library and its screening technology.
KSV peptides is good at combined screening way by unifying OBOC and DEL which contributes to the accuracy of screening.
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