SEARCH
ENGLISH
CAT | H3116 |
Product Name | Histone H3-K9me3-K18Ub-K23Ub |
Purity | ≥95% by HPLC analysis |
Appearance | White powder |
Usage | Research use only |
Storage | Shipping at room temperature. Upon delivery aliquot. Stored at -20°C or -80°C. Avoid freeze/thaw cycle. |
The DNMT1 RFTS domain serves as an effector module that transmits the H3K9me3 signal into DNMT1-mediated DNA methylation. DNMT1 RFTS domain binds to histone H3 ubiquitinated at lysine 14 (K14Ub), 18 (K18Ub), and/or 23 (K23Ub), with a preference for H3 with two mono-ubiquitination (H3Ub2). Dnmt1 is inhibited by its RFTS domain, which binds the catalytic domain to the exclusion of DNA. Activation of Dnmt1 at specific times and locations is crucial for the maintenance of proper DNA methylation during cell proliferation, which ensures tissue- or cell-type-specific functions. Thus, constitutively active Dnmt1 leads to abnormal methylation patterns that affect this cell-type-specific functional differentiation. the binding of H3 doubly monoubiquitinated at K18 and K23 to RFTS leads to Dnmt1 activation through dissociation of inserted RFTS from the catalytic domain.
English 