Peptide solid-phase synthesis is a major breakthrough in peptide synthesis chemistry. Its biggest feature is that it is not necessary to purify the intermediate products, and the synthesis process can be carried out continuously, which in turn lays the foundation for the automation of peptide synthesis. At present, the fully automated peptide synthesis is basically solid-phase synthesis. The basic process is as follows: based on Fmoc chemical synthesis, the carboxyl group of the C-terminal amino acid of the target peptide to be synthesized is first linked to an insoluble polymer resin in the form of a covalent bond, and then the amino group of this amino acid is used as the starting point of peptide synthesis, and the peptide bond is formed with the activated carboxyl group of other amino acids, and this process is repeated continuously to obtain the peptide. Depending on the amino acid composition of the peptide, the peptide post-processing method is different and the purification method is also different.
The KS-V Peptide are produced strictly according to ISO quality management system, and each peptide is managed with unique number, and the crude product, purified collection solution and final lyophilized product are analyzed by HPLC as well as LC/MS to ensure the correctness of the product and the quality of the product.
Peptide purification usually uses a reversed-phase column (such as C8, C18, etc.), 214nm. the buffer system is usually solvent containing TFA, pH2.0. buffer A is 0.1% TFA in ddH2O, buffer B is 1% TFA/ACN/pH2.0. dissolve with Buffer A before purification; if dissolution is not good, dissolve with Buffer B and then dilute with Buffer A. for hydrophobic peptides, sometimes a small amount of Formic Acid or acetic acid is needed. HPLC analysis of crude peptide products, if the peptide is not long (below 15aa), there is usually the main peak, and the main peak is usually the full-length product; for long peptides above 20aa, if there is no main peak, HPLC needs to be paired with Mass to determine the molecular weight, and then determine which peak is the peptide to be synthesized.
KS-V Peptide has accumulated many international leading and difficult peptide synthesis technologies in peptide chemical synthesis technology, and can realize the chemical synthesis of the long-chain peptide with the help of peptide fragment synthesis and peptide linkage technology; we have mastered the solubilization technology of highly hydrophobic membrane protein fragment synthesis, and completely applied chemical synthesis method to realize the longest membrane protein chemical total synthesis in the world at present, which has set a new world record for many times; and we have applied alternative disulfide-bonded non-natural amino acid technology to achieve the peptide cyclization modification, which will make the synthesized peptide drug more stable. Common peptides can be delivered in one week's time, and most of them can be delivered in two weeks. Quantities range from milligrams to kilograms.