A peptide library is a large collection of synthetic peptides with systematic amino acid combinations. These synthetic peptide library screening tools are used for epitope peptide mapping, peptide library screening for drug target validation, and vaccine development. A typical peptide library contains up to 25 amino acids. However, we can synthesize long chain peptides up to 400 amino acids to ensure coverage of potential proteinaceous peptides that need to be screened for SRM assays.
Peptide libraries are synthesized on our proprietary high-throughput combinatorial peptide synthesis platform. Final unbound peptides are carefully placed in separate vials to avoid cross-contamination, or packaged in 96-well tube plates.
With the advent of combinatorial chemistry and the establishment of automated solid-phase synthesis techniques, it became possible to synthesize a large number of peptides in large quantities. These libraries can be used in the development of therapeutics as well as in the design of peptide libraries to allow their activity to be screened using various combinations of screening techniques such as epitope mapping, etc.
By combining different resins with linker molecules and coupling methods, various libraries can now be synthesized. After synthesis, libraries can be cleaved from resin to generate peptide libraries in solution that can be used in various formats or spotted onto supports such as membranes made of cellulose, pvdf, or coated glass slides. In addition, peptide libraries can also be left on resin or beads after synthesis. The use of defined linkers has proven beneficial for peptide libraries on solid supports.
Solid-phase peptide synthesis has been successfully used to prepare libraries for the following applications: antibody epitope mapping and validation to determine antibody specificity; identification of bioactive peptides; development of bioassays; T cell epitope mapping; vaccine efficacy testing; Screening for ligand binding activity; screening for antimicrobial peptide activity; screening for peptide-protein interactions; drug discovery libraries; libraries for affinity optimization; peptide libraries for LC-MS/MS method development and validation; Libraries for in vivo studies and cellular analysis; libraries containing restricted peptides; libraries containing modified peptides, such as histone peptides; screening for MHC class I and II, and many others.
Types of peptide libraries are on-resin or off-resin libraries, libraries containing peptides with scrambled amino acid residues, peptide libraries containing specific linker molecules, dye-labeled peptide libraries, specially modified libraries, multi-epitope peptide libraries for vaccine development , other combinatorial libraries.