Peptide synthesis, also known as peptide chain synthesis, is a solid-phase synthesis sequence generally from the C-terminus (carboxy-terminus) to the N-terminus (amino-terminus). In the past, peptide synthesis was carried out in solution called liquid-phase synthesis. Peptide synthesis is mainly divided into two pathways: chemical synthesis of peptides and biosynthesis of peptides. Polypeptide synthesis is how to connect various amino acid units according to the amino acid sequence and connection method of natural products.
Increased research speed: Another significant advantage of custom peptide synthesis is to simplify the overall process and increase research throughput. These mechanized workstations help to assess the sample size in a given area to increase the sample size. As a result, the overall reliability of the experiment is improved.
Certain automated microarray scanners, such as the VERSA110, include four SBS platform positions to support more important studies.
Increased productivity: As throughput increases and the risk of errors decreases, so does overall productivity. When experiments have tighter tolerances, analysts can move forward with projects faster and with better use of their time and expertise. Furthermore, when using a custom peptide synthesis program, the analyst may leave the protocol knowing that it will perform the task quickly and correctly.
Eliminating unwanted volatile compounds is simple: in custom peptide synthesis, a retrophase peptide is one in which the initial amino acid in the sequence binds to the substrate. Anything attached to the polymer becomes inaccessible, making it easier to distinguish soluble molecules from those that can be removed. The necessary chemical interaction between the open-flow amino acid and the current chain is also more direct with the peptide linked to the polymer. The chain stays in place, reducing unpredictability in the water system.
The order of reactions can be controlled: chemical groups are added to free amino acids to prevent them from interacting with other compounds in the system. A number of chemical groups are used, each of which can be eliminated using a different chemical procedure. This allows scientists to choose which amino acids are created first to respond, which amino acids are made reactive, and so on. Only the next required amino acid in the sequence is attached to the tethered chain using this method.